Browsing by Subject "Autofluorescence"

Now showing 1 - 4 of 4
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    Autofluorescence spectroscopy of malpighian epithelial cells, as a new tool for analysis of cervical cancer precursors
    (Murcia : F. Hernández, 2003) Millot, C.; Bondza-Kibangou, P.; Millot, J.M.; Lallemand, A.; Manfait, M.
    A spectroscopic analysis of autofluorescence was investigated within the cell cytoplasm from cervical malpighian epithelia prepared on Thin-Prep smears. Autofluorescence emission spectra from 22 cervix were analyzed by microspectrofluorometry under a 363 nm laser excitation. Among the analyzed cervix, 6 were in normal limits, 6 in inflammatory limits, 5 were evocative of Low-Grade Squamous Intraepithelial Lesions (LGSILs) and 5 were evocative of High-Grade Squamous Intraepithelial Lesions (HGSILs). Cytoplasmic emission intensities at 450 nm of cells from inflammatory, LGSIL and HGSIL cervix were equivalent and were 3-fold higher than from normal cervix. All smears presented a two-fold lower autofluorescence emission in the cytoplasm than in the nucleus. The spectral profile analysis allows the discrimination of cells from inflammatory, LGSIL and HGSIL cervix. The 525/425 nm emission ratios were 0.75±0.1, 0.96±0.04 and 1.2±0.1 for inflammatory, LGSIL and HGSIL, respectively. We suggest that smears of normal, inflammatory, LGSIL and HGSIL cervix could be discriminated by the analysis of the 450 nm emission intensity and 525/425 nm emission ratios from cells of malpighian epithelia.
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    Eosin-related fluorescence of acidophil pituitary cells
    (Murcia : F. Hernández, 1988) Friedman, H.; Friedman, I.V.C.; Mello, C.V.
    The examination of haematoxylin and eosin stained sections of normal and neoplastic pituitary glands under ultraviolet light illumination discloses fluorescence of acidophil cells. The distinction between prolactin and growth hormone-producing cells is not possible. Such fluorescence depends on previous eosin staining.
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    Predictive value of a non‑invasive autofluorescence‑based device (GOCCLES®) in the early diagnosis of oral potentially malignant disorders and oral cancer: a cross‑sectional study
    (Springer, King Saud University, 2025-11-22) Martín‑Muñoz, Rocío Teresa; Rodríguez‑Molinero, Jesús; Migueláñez‑Medrán, Blanca del Carmen; Ruiz Roca, Juan Antonio; López Jornet, María Pía; Ramírez‑Puerta, Rosario María; López‑Sánchez, Antonio Francisco; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
    Purpose: Oral cancer is the sixteenth most common malignancy globally and is characterized by a 5-year survival rate of less than 40%. In recent years, innovative methods have been developed to facilitate its early diagnosis, including the GOCCLES® device. This study aimed to evaluate the diagnostic value of GOCCLES® in detecting dysplasia in oral potentially malignant disorders (OPMDs) and diagnosing oral squamous cell carcinoma (OSCC). Additionally, this study sought to compare the device’s efficacy with toluidine blue (TB) staining and assess the value of combining both methods. Methods: Patients clinically diagnosed with OPMDs or OSCC were included. Anamnesis, oral examination, inspection with GOCCLES®, and TB staining were performed. Finally, lesions were biopsied and the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of each technique were determined. Results: Thirty-two patients were included in this study. The GOCCLES® device had a sensitivity of 33.3%, specificity of 40%, PPV of 25%, NPV of 50%, and accuracy of 37.5%. For TB staining, the sensitivity, specificity, PPV, NPV, and accuracy were 58.3%, 85%, 70%, 77.27%, and 75%, respectively. The respective values for the combined method were 44.4%, 77.78%, 66.67%, 58.33%, and 61.11%. Conclusions: GOCCLES® and TB staining could help in the early diagnosis of OSCC and OPMDs, but a biopsy of the lesion remains essential for obtaining a definitive diagnosis.
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    Sudan Black B treatment reduces autofluorescence and improves resolution of in situ hybridization specific fluorescent signals of brain sections
    (Murcia: F. Hernández, 2010) Oliveira, V.C.; Carrara, R.C.V.; Simoes, D.L.C.; Saggioro, F.P.; Carlotti, C.G.; Covas, D.T., Jr.; Neder, L.
    Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels.