Browsing by Subject "Atmosphere"

Now showing 1 - 3 of 3
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    Distribution of airborne pollen, fungi and bacteria at four altitudes using high-throughput DNA sequencing
    (Elsevier, 2020-10-11) Sánchez-Parra, Beatriz; Núñez, Andrés; García, Ana M.; Campoy, Pascual; Moreno, Diego A.; Genética y Microbiología
    The diversity and composition of bioaerosols in the atmosphere have been studied in the recent years in multiple places and under very different meteorological conditions. Pollen grains, fungal propagules and bacteria are the most studied airborne biological particles, usually individually, and correlated to environmental factors such as temperature, wind speed, rain, air pollution, etc. to understand their influence. However, the effect of the height on the distribution and composition of these bioaerosols has not been properly described so far. In this research, we analyzed concurrently these three biological particles at four different heights (1.5 m, 130 m, 500 m and 1000 m) by high-throughput DNA sequencing. We observed that the atmosphere contains a homogeneous distribution and composition of biological particles regardless of the altitude tested. Only a minority of the taxa is differently distributed. Therefore, we here deduce that the altitude does not drastically influence the airborne bioparticles distribution and composition under similar meteorological conditions. We also studied the co-occurrence between different bioaerosols, finding new potential relationships.
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    Monitoring of airborne biological particles in outdoor atmosphere. Part 1: Importance, variability and ratios
    (Hemeroteca Científica Catalana [University Publisher], 2016-03-10) Núñez, Andrés; Amo de Paz, Guillermo; Rastrojo, Alberto; García, Ana M.; Alcamí, Antonio; Gutiérrez-Bustillo, A. Montserrat; Moreno, Diego A.; Genética y Microbiología
    The first part of this review ("Monitoring of airborne biological particles in outdoor atmosphere. Part 1: Importance, variability and ratios") describes the current knowledge on the major biological particles present in the air regarding their global distribution, concentrations, ratios and influence of meteorological factors in an attempt to provide a framework for monitoring their biodiversity and variability in such a singular environment as the atmosphere. Viruses, bacteria, fungi, pollen and fragments thereof are the most abundant microscopic biological particles in the air outdoors. Some of them can cause allergy and severe diseases in humans, other animals and plants, with the subsequent economic impact. Despite the harsh conditions, they can be found from land and sea surfaces to beyond the troposphere and have been proposed to play a role also in weather conditions and climate change by acting as nucleation particles and inducing water vapour condensation. In regards to their global distribution, marine environments act mostly as a source for bacteria while continents additionally provide fungal and pollen elements. Within terrestrial environments, their abundances and diversity seem to be influenced by the land-use type (rural, urban, coastal) and their particularities. Temporal variability has been observed for all these organisms, mostly triggered by global changes in temperature, relative humidity, et cetera. Local fluctuations in meteorological factors may also result in pronounced changes in the airbiota. Although biological particles can be transported several hundreds of meters from the original source, and even intercontinentally, the time and final distance travelled are strongly influenced by factors such as wind speed and direction.
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    Validation of the hirst-type spore trap for simultaneous monitoring of prokaryotic and eukaryotic biodiversities in urban air samples by next-generation sequencing
    (American Society for Microbiology, 2017-04-28) Núñez, Andrés; Amo de Paz, Guillermo; Ferencova, Zuzana; Rastrojo, Alberto; Guantes, Raúl; García, Ana M.; Alcamí, Antonio; Gutiérrez-Bustillo, A. Montserrat; Moreno, Diego A.; Genética y Microbiología
    Pollen, fungi, and bacteria are the main microscopic biological entities present in outdoor air, causing allergy symptoms and disease transmission and having a significant role in atmosphere dynamics. Despite their relevance, a method for monitoring simultaneously these biological particles in metropolitan environments has not yet been developed. Here, we assessed the use of the Hirst-type spore trap to characterize the global airborne biota by high-throughput DNA sequencing, selecting regions of the 16S rRNA gene and internal transcribed spacer for the taxonomic assignment. We showed that aerobiological communities are well represented by this approach. The operational taxonomic units (OTUs) of two traps working synchronically compiled >87% of the total relative abundance for bacterial diversity collected in each sampler, >89% for fungi, and >97% for pollen. We found a good correspondence between traditional characterization by microscopy and genetic identification, obtaining more-accurate taxonomic assignments and detecting a greater diversity using the latter. We also demonstrated that DNA sequencing accurately detects differences in biodiversity between samples. We concluded that high-throughput DNA sequencing applied to aerobiological samples obtained with Hirst spore traps provides reliable results and can be easily implemented for monitoring prokaryotic and eukaryotic entities present in the air of urban areas.