Browsing by Subject "Anoxia"

Now showing 1 - 3 of 3
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    A histopathological study of anoxic-resuscitated liver allografts
    (Murcia : F. Hernández, 1997) Jurado, F.; Buján, J.; Mora, M.P.; Jiménez, M.; Arahuetes, Rosa María; Bellón, J.M.
    The possibility of resuscitating livers after warm ischaemia has been recently suggested. The aim of the present investigation was to analyse the effects of anoxia on the morphology of hepatic cells, to determine whether these effects are reversible after providing a resuscitation period between warm ischaemia (WI) and cooling, and to study the behaviour of the resuscitated liver in the recipient organism. Ten female, Large-White pigs acted as donors for 10 recipient animals of the same kind who received an orthotopic liver graft. Recipients were divided into two groups depending on whether the livers they received had undergone a resuscitation period (Group 1 (n=5) where animal livers were subjected to 5 min warm ischaemia (WI) without resuscitation, and Group 11 (n=5) where the livers were subjected to 5 min WI followed by 5 min resuscitation). Morphological and ultrastructural studies of liver cells were performed using light and electron microscopy. ATP, ADP and AMP levels were determined in liver biopsies by high performance liquid chromatography (HPLC). Plasma AST and bilirubin levels in the two groups were compared 24 h after transplantation. After 5 min of anoxia, hepatocytes showed two morphological pattems in response to WI. Some were appreciably condensed with dark mitochondria, peroxisomes and some cytoplasmic vacuoles. Others showed electronlucent organelles, inflamed mitochondria with broken cristae and disorganized endoplasmic reticulum. Hepatocytes showed globular microvilli and bleb formation with migration towards the sinusoids. One hour after the revascularisation of the resuscitated livers, the hepatocytes showed nearly normal morphological characteristics. However, the hepatocytes of nonresuscitated organs continued to show alterations. Kupffer cells were activated in the livers of both experimental groups. Ultrastructural changes and total tissue adenine nucleotide (TAN) levels recovered completely in resuscitated livers soon after transplant. These results suggest that when short WI periods are followed by equivalent periods of resuscitation, the hepatocytes of transplanted livers recover from the effects of anoxia.
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    Environmental Crisis in the Mar Menor Lagoon (Región de Murcia-Spain)
    (Associaçäo Brasileira de Editores Científicos, 2024-11-27) Gil Meseguer, Encarnación; Bernabé Crespo, Miguel Borja; Gómez Espín, José María; Geografía
    Objective: The objective of this study is to gain insight into the causes of the environmental crisis that affects the Mar Menor ecosystem and its surroundings, as well as to propose measures for a new territorial model. Theoretical Framework: It is based on the study of population growth in the Campo de Cartagena - Mar Menor region, as well as the different activities that take place there, especially focusing on regenerated water. Method: The methodology adopted for this research includes regional geographic analysis for the management of the territory, with extensive field work and interviews with users of the Mar Menor; to conclude with a series of “zero discharge” proposals and cultural practices to mitigate the environmental crisis of the lagoon and its surroundings. Results and Discussion: Agriculture, urban expansion, obsolescence of sanitation networks, and other activities such as stable livestock farming and mining waste hauling have caused anthropization of the Mar Menor with eutrophication phenomena. It is advisable to promote a bottom-up participatory process of citizens, reach agreements between Administrations, and harmonize the different land uses making them compatible with the good condition of the lagoon. Research Implications: The practical and theoretical implications of this research are discussed, discussing the causes of the state of the lagoon and the uses of the catchment area. Originality/Value: This study contributes to the literature by addressing a topic of interest and concern from a comprehensive perspective, considering causes and consequences, as well as possible solutions. The relevance and value of this research are evident in its geographical approach to human-natural environment relationships.
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    Environmental crisis in the Mar Menor Lagoon (Region of Murcia - Spain)
    (Interinstitutional Scientific Committee, 2024-11-27) Gil-Meseguer, Encarnación; Bernabé-Crespo, Miguel Borja; Gómez Gil, José María; Gómez Espín, José María; Geografía
    Objective:The objective of this study is to gain insight into the causes of the environmental crisis that affects the Mar Menor ecosystem and its surroundings, as well as to propose measures for a new territorial model.Theoretical Framework:It is based on the study of population growth in the Campo de Cartagena -Mar Menor region, as well as the different activities that take place there, especially focusing on regenerated water.Method:The methodology adopted for this research includes regional geographic analysis for the management of the territory, with extensive field work and interviews with users of the Mar Menor; to conclude with a series of “zero discharge” proposals and cultural practices to mitigate the environmental crisis of thelagoon and its surroundings.Results and Discussion:Agriculture, urban expansion, obsolescence of sanitation networks, and other activities such as stable livestock farming and mining waste hauling have caused anthropization of the Mar Menor with eutrophication phenomena. It is advisable to promote a bottom-up participatory process of citizens, reach agreements between Administrations, and harmonize the different land uses making them compatible with the good condition of the lagoon.Research Implications:The practical and theoretical implications of this research are discussed, discussing the causes of the state of the lagoon and the uses of the catchment area.Originality/Value:This study contributes to the literature by addressing a topic of interest and concern from a comprehensive perspective, considering causes and consequences, as well as possible solutions. The relevance and value of this research are evident in its geographical approach to human-natural environment relationships. -------------------