Browsing by Subject "Anisakis"

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    Molecular identification of Anisakis and Hysterothylacium larvae incommercial cephalopods from the Spanish Mediterranean coast
    (Elsevier, 2016-04-15) Picó-Durána, Gabriela; Pulleiro-Potela, Lorena; Abolloc, Elvira; Pascual, Santiago; Muñoz, Pilar; Sanidad Animal
    This study aims to investigate the occurrence of nematode larvae in commercial cephalopods in theWestern Mediterranean Sea. A total of 202 animals comprising 123 broadtail shortfin squid (Illex coinde-tii), 34 European squid (Loligo vulgaris) and 45 common octopus (Octopus vulgaris) were examined usingenzymatic digestion. A total of 31 larvae were isolated (prevalence: 14.6%) and identified using molecularanalyses which included PCR and sequencing of the ITS (ITS1-5.8S rDNA-ITS2) region. Phylogenetic treeinferred from ITS sequences yielded supported relationships for Anisakis (P: 12.2%) and Hysterothylaciumspecies (P: 4.1%). All parasites were found parasitizing I. coindetii and, as expected, A. pegreffii presentedthe highest prevalence (11.4%). A. physeteris was also found with a lower prevalence (1.6%) but confirmingthe role of the broadtail shortfin squid as paratenic host and, its potential host for anisakidosis transmis-sion. A hybrid larva between Anisakis simplex and A. pegreffi was also identified. All Anisakis larvae werefound within the visceral cavity; in contrast most of the Hysterothylacium larvae were isolated from themantle. A significant correlation was found between total nematode prevalence and depth, explained bythe presence of larger broadtail shortfin squids inhabiting deeper depths. Therefore, the results obtainedin the present study improve the knowledge of the occurrence of Anisakis and Hysterothylacium species inthe I. coindetii from the Spanish Mediterranean Sea highlighting the importance of considering I. coindetiias a potential hazard for humans if it is consumed raw or not well cooked and the need of further researchin other cephalopods.
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    Occurrence of Anisakis and Hysterothylacium larvae in commercial fish from Balearic Sea (Western Mediterranean Sea)9
    (Springer, 2018-10-16) Barcala, Elena; Ramilo, Andrea; Picó, Gabriela; Abollo, Elvira; Pascual, Santiago; Muñoz, Pilar; Ortega Hernández, Nieves; Sanidad Animal
    This study investigates the occurrence of anisakids and raphidascarids in commercial fish from Balearic Sea (Western Mediterranean). A total of 335 fish including 19 black anglerfish (Lophius budegassa), 33 white anglerfish (L. piscatorius), 129 European hake (Merluccius merluccius), 30 red mullet (Mullus barbatus), and 124 striped mullet (M. surmuletus) were examined using enzymatic digestion. A total of 948 nematode larvae were isolated (prevalence 52.53%) being the highest prevalence observed in striped mullet. Forty-six larvae were identified using molecular analyses which included PCR and sequencing of the 629-bp fragment of mitochondrial cox2 gene region. Anisakis pegreffii (80.43%), A. physeteris (8.69%), Hysterothylacium fabri (6.52%), and A. simplex (4.35%) were detected based on molecular analyses of larvae. Total nematode prevalence was positively correlated with weight, length, condition factor, and maturity stage of the host and also with fishing ground depth. Statistical differences between total nematode prevalence and geographical sector of capture were observed when fishing hauls were grouped according to the abundance of sperm whales or common bottlenose dolphins. The results also corroborate that fishing water depth may play an important role in anisakid and raphidascarid parasitization.1SPS� m