Browsing by Subject "Alternative methods"

Now showing 1 - 2 of 2
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    A comparison of BGM and LLC-PK1 cells for the evaluation of nephrotoxicity
    (Taylor and Francis Group, 2011-09-22) Tagliati, Carlos A.; Romero García, Diego; Dutra, Elisabete C.R.; Hernández-García, Alejandro; Martínez-López, Emma; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    Nephrotoxicity is one of the most frequent effects observed after the use of medicine. Such situations have been tardily discovered because of existing methods to determine toxicity. The validation of sensitive, alternative methods for the early identification of toxic effects is as important as restrictions on the use of animals. In this light, the present study evaluated the effects of gentamicin on BGM and LLC-PK1 cells, using MTT and Neutral Red (NR). Although the LLC-PK1 cell line is used for toxicological studies, the BGM cell line is relatively new for this purpose. MTT (BGM: EC50=6.29mM; LLC-PK1: EC50=8.01mM) was found to be more sensitive than NR (EC50 was greater than 10mM for both cells). By using MTT, both cells demonstrated the involvement of mitochondria in a manner that was dose dependent, with an apoptotic process occurring at the concentrations of 1 and 3mM and necrosis at concentrations above 4mM. It could, therefore, be concluded that 1) BGM appears to be useful in the study of the mechanism of nephrotoxicity caused by gentamicin and 2) because of its sensitivity to MTT, in addition to its ease of manipulation, it is believed that the BGM cell line can also be used as an alternative method to evaluate nephrotoxicity.
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    Ex vivo applications of porcine ocular surface tissues: Advancing eye research and alternatives to animal studies
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Diebold, Yolanda; García Posada, Laura; Biología Celular e Histología
    The use of tissues of porcine origin has gained significant momentum in the scientific community due to their anatomical and physiological resemblance to human tissues. This review provides a comprehensive overview of the key biological features of porcine ocular structures, including the cornea, conjunctiva, and associated tissues, in comparison to their human counterparts. Additionally, this review outlined the ex vivo applications of these tissues in the study of different biological processes and the simulation of pathological conditions. By high-lighting the potential of porcine ocular surface tissues as cost-effective, ethically appropriate, and reliable models, this review underscores their value in advancing eye and vision research.