Browsing by Subject "Adrenal"

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    lmmunohistochemical analysis of adrenal proliferation and corticosterone expression in experimental adrenal regeneration
    (Murcia : F. Hernández, 1995) Sarría, R.; Losada, J.; Bueno-López, J.L.
    The proliferative activity, the organization and the corticosterone expression of adrenocortical cells in an experimental adrenal regeneration process after the transplantation of neonatal adrenal glands to adult hosts was investigated. Three days after transplantation, the medullar and the innermost adrenocortical cells of the neonatal adrenal glands showed degenerative and necrotic changes due to the lack of vascular supply. The remaining outermost adrenocortical cells did not display any PCNA immunoreaction. The first PCNA expression, pointing out the beginning of the proliferative cycle, was observed in a 45.4% of the adrenocortical cells, one week after transplantation. After three weeks, severa1 regenerated adrenocortical nodules with a bigger size than the one observed in the previous periods were seen. In these nodules, while the outermost adrenocortical cells were disposed in parallel to the capsule or in rounded groups, the bulk of the regenerated mass width was composed of cells forming longitudinal cords. PCNA immunoreaction was almost exclusively restricted to subcapsular cells (62.5%) and to cells of the outermost portion of the cords (32.5%), the global percentage of PCNA immunopositive cells being 18.4%. Twelve weeks after transplantation, regenerated adrenocortical cells were arranged in three layers: glomerulosa, fasciculata and reticularis. Only 1.85% of the adrenocortical cells were PCNA immunopositive. Although in the early stages of the regeneration process, al1 the adrenocortical cells, both proliferating and non proliferating cells expressed corticosterone, a restriction of this immunoreactivity to the zonae fasciculata and reticularis was observed when cell zonation was apparent.
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    Nuclear bodies in the normal and hyperfunctional human adrenal cortex
    (Murcia : F. Hernández, 1989) Magalhães, M. M.; Carvalho, D.; Oliveira, M. A.; Magalhães, M. C.
    Adrenal pieces obtained from six female patients, three without increased adrenocortical function and three with cushing's disease, showed, in all adrenal cortex zones, cells containing simple and complex nuclear bodies. The simple nuclear bodies were spherical or ovoid and had a filamentous structure surrounded by a clear halo. Complex nuclear bodies were more numerous and heterogeneous in patients with adrenal pathology, and they were spherical with a proteinaceous filamentous capsule surrounding a core; the core was granular, filamentous or a mixture of granular and filamentous material, sometimes with a reticular or concentric arrangement. Some bodies showed vacuolar or multilocular aspect, and others had a close relationship with the nucleolus or appeared near the interchromatin granules.