Browsing by Subject "AKT"

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    7,8-Dihydroxiflavone protects adult rat axotomized retinal ganglion cells through MAPK/ERK and PI3K/AKT activation
    (MDPI, 2021-10-08) Galindo Romero, Caridad; Vidal-Villegas, Beatriz; Asís-Martínez, Javier; Lucas Ruiz, Fernando; Gallego Ortega, Alejandro; Vidal Sanz, Manuel; Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica; Facultad de Óptica y Optometría
    We analyze the 7,8-dihydroxyflavone (DHF)/TrkB signaling activation of two main intracellular pathways, mitogen-activated protein kinase (MAPK)/ERK and phosphatidylinositol 3 kinase (PI3K)/AKT, in the neuroprotection of axotomized retinal ganglion cells (RGCs). Methods: Adult albino Sprague-Dawley rats received left intraorbital optic nerve transection (IONT) and were divided in two groups. One group received daily intraperitoneal DHF (5 mg/kg) and another vehicle (1%DMSO in 0.9%NaCl) from one day before IONT until processing. Additional intact rats were employed as control (n = 4). At 1, 3 or 7 days (d) after IONT, phosphorylated (p)AKT, p-MAPK, and non-phosphorylated AKT and MAPK expression levels were analyzed in the retina by Western blotting (n = 4/group). Radial sections were also immunodetected for the above-mentioned proteins, and for Brn3a and vimentin to identify RGCs and Müller cells (MCs), respectively (n = 3/group). Results: IONT induced increased levels of p-MAPK and MAPK at 3d in DHF- or vehicle-treated retinas and at 7d in DHF-treated retinas. IONT induced a fast decrease in AKT in retinas treated with DHF or vehicle, with higher levels of phosphorylation in DHF-treated retinas at 7d. In intact retinas and vehicle-treated groups, no p-MAPK or MAPK expression in RGCs was observed. In DHF- treated retinas p-MAPK and MAPK were expressed in the ganglion cell layer and in the RGC nuclei 3 and 7d after IONT. AKT was observed in intact and axotomized RGCs, but the signal intensity of p-AKT was stronger in DHF-treated retinas. Finally, MCs expressed higher quantities of both MAPK and AKT at 3d in both DHF- and vehicle-treated retinas, and at 7d the phosphorylation of p-MAPK was higher in DHF-treated groups. Conclusions: Phosphorylation and increased levels of AKT and MAPK through MCs and RGCs in retinas after DHF-treatment may be responsible for the increased and long-lasting RGC protection afforded by DHF after IONT.
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    FAM107A inhibits invasion and migration of colorectal cancer by affecting EMT via the AKT pathway
    (2026) Zhenyu Dai; Wenhui Li; Jun Zhu; Chunlong Li; Rui Huang; Haoguang Wan; Yue Liu; Lizhou Chen; Biología Celular e Histología; Universidad de Murcia, Departamento de Biologia Celular e Histiologia
    Metastasis of colorectal cancer (CRC) is the main cause of CRC-related mortality. FAM107A is widely expressed in various normal tissues. However, few studies have revealed the biological function of FAM107A in epithelial-mesenchymal transition (EMT) in human cancer cells, and the related molecular mechanisms and signaling cascades are completely unknown. Here, we found that FAM107A was abnormally expressed in human CRC tissues and cell lines. Further research has shown that overexpression of FAM107A through transfection weakened the expression level of EMT-related markers. In addition, our research results indicated that upregulation of FAM107A inhibited the AKT signaling cascade in human CRC cells, while AKT activators restored activation of p50, indicating that FAM107A may regulate EMT through AKT activation of p50. Our results suggest that FAM107A could be a potential target for the treatment of CRC.