(Universidad de Murcia, Servicio de Publicaciones, 1985) Sánchez Bravo, José; Ortuño Tomás, Ana María; Acosta Echevarría, Manuel; Sabater, F.; Facultad de Biología
The quantification of the phytohormone, indolil-3-acetic acid l]AAJ. in me.thanolic extracts of etiolated
hypocotyls of Lupíne by a fluorimetric method, has been investigated. The applícation of the established
criteria for the use of thís method shows that the observed fluorescence corresponds to the fluorescent lAA
derivative. and that the other components of the methanolic extracts do not interfere in the measure. So, it is
possible to quantify directly the !AA in the methanolic extracts without previous purification.